Graduate School of Medicine, Kansai Medical University
The project aims to develop standardized methods for analyzing the formation, degeneration and restoration of human brain tissues. Combining 3D brain organoid cultures of human pluripotent stem cells, 4D imaging and image analysis, we establish efficient methods to faithfully recapitulate and to quantitatively analyze the ontogenetic formation of human brain tissues. We induce degeneration of the formed tissues to construct human neurological disease models. We further explore the ways to prevent or restore the degeneration for clinical treatment and drug discovery.